anti mouse p stat 1 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti mouse p stat 1

Sauchinone inhibits M1 macrophage polarization and promotes M2 macrophage polarization in BMDM. (A) The cytotoxicity of Sauchinone on BMDM was detected by CCK-8 kit. (B-G) BMDM were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of different doses of Sauchinone. The transcription levels of Tnf-α , Il-6 , Il −12 , Inos , Il-1β , Cox-2 and Nlrp3 (B) were detected by RT-qPCR. TNF-α, IL-6, IL-12 and NO levels in culture supernatants (C) were measured by ELISA or Griess assay. The protein levels of <t>p-STAT-1,</t> STAT-1, and INOS in cell lysates (D) were detected by western blot. The percentages of INOS + , MHCⅡ + and CD86 + BMDM (E) were measured by FACS. (F-H) BMDM were stimulated with IL-4 plus IL-13 to induce M2 polarization in the presence or absence of different doses of Sauchinone. The transcription levels of Arg-1, Fizz1 , Ym-1 , Cd206 , Cd301 , and Dectin-1 (F) were detected by RT-qPCR. The protein levels of p-STAT-6 and STAT-6 in cell lysates (G) were detected by western blot. The percentages of CD301 + BMDM (H) were measured by FACS. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Anti Mouse P Stat 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+stat+1/Phospho-Stat1+(Tyr701)+Rabbit+mAb/pmc12869217-66-3-49
Average 96 stars, based on 545 article reviews

anti mouse p stat 1 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization"

Article Title: A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization

Journal: Journal of Advanced Research

doi: 10.1016/j.jare.2025.04.034

Figure Legend Snippet: Sauchinone inhibits M1 macrophage polarization and promotes M2 macrophage polarization in BMDM. (A) The cytotoxicity of Sauchinone on BMDM was detected by CCK-8 kit. (B-G) BMDM were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of different doses of Sauchinone. The transcription levels of Tnf-α , Il-6 , Il −12 , Inos , Il-1β , Cox-2 and Nlrp3 (B) were detected by RT-qPCR. TNF-α, IL-6, IL-12 and NO levels in culture supernatants (C) were measured by ELISA or Griess assay. The protein levels of p-STAT-1, STAT-1, and INOS in cell lysates (D) were detected by western blot. The percentages of INOS + , MHCⅡ + and CD86 + BMDM (E) were measured by FACS. (F-H) BMDM were stimulated with IL-4 plus IL-13 to induce M2 polarization in the presence or absence of different doses of Sauchinone. The transcription levels of Arg-1, Fizz1 , Ym-1 , Cd206 , Cd301 , and Dectin-1 (F) were detected by RT-qPCR. The protein levels of p-STAT-6 and STAT-6 in cell lysates (G) were detected by western blot. The percentages of CD301 + BMDM (H) were measured by FACS. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Techniques Used: CCK-8 Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Griess Assay, Western Blot

Sauchinone inhibits macrophage M1 polarization in TGR5 dependent manner. (A-B) BMDM from WT mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM) or SBI-115 (5 μM). The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (A) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (B) were detected by western blot. (C-E) BMDM from WT mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM) or TGR5 si-RNA. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (C) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (D) were detected by western blot. IL-6 and NO levels in culture supernatants (E) were measured by ELISA and Griess assay. (F-H) BMDM from Tgr5 -/- mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM). IL-6 and NO levels in culture supernatants (F) were measured by ELISA or Griess assay. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (G) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (H) were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Figure Legend Snippet: Sauchinone inhibits macrophage M1 polarization in TGR5 dependent manner. (A-B) BMDM from WT mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM) or SBI-115 (5 μM). The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (A) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (B) were detected by western blot. (C-E) BMDM from WT mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM) or TGR5 si-RNA. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (C) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (D) were detected by western blot. IL-6 and NO levels in culture supernatants (E) were measured by ELISA and Griess assay. (F-H) BMDM from Tgr5 -/- mice were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of Sauchinone (30 μM). IL-6 and NO levels in culture supernatants (F) were measured by ELISA or Griess assay. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (G) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (H) were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Techniques Used: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Griess Assay

Sauchinone inhibits macrophage M1 polarization through TGR5/PKA pathway. (A-C) BMDM were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of different doses of Sauchinone (30 μM) or PKA inhibitor H89 (10 μM). IL-6 and NO levels in culture supernatants (A) were measured by ELISA or Griess assay. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (B) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (C) were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Figure Legend Snippet: Sauchinone inhibits macrophage M1 polarization through TGR5/PKA pathway. (A-C) BMDM were stimulated with LPS plus IFN-γ to induce M1 polarization in the presence or absence of different doses of Sauchinone (30 μM) or PKA inhibitor H89 (10 μM). IL-6 and NO levels in culture supernatants (A) were measured by ELISA or Griess assay. The transcription levels of Tnf-α , Il-6 , Inos and Il-1β (B) were detected by RT-qPCR. The protein levels of p-STAT-1, STAT-1 and INOS in cell lysates (C) were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Techniques Used: Enzyme-linked Immunosorbent Assay, Griess Assay, Quantitative RT-PCR, Western Blot

Sauchinone normalizes TGR5/PKA/CREB pathway and regulates M1/M2 macrophages markers in skin of IMQ-induced psoriasis mice. (A) The protein levels of TGR5, PKA. p-CREB, CREB in skin tissues were detected by western blot. (B) Cytokines including IL-6, IL-12 TNF-α, IFN-γ, IL-17 and IL-10 levels in serum were measured by ELISA (n = 6). (C) Cytokines including IL-6, IL-12, TNF-α, IFN-γ, IL-1β and IL-10 levels in skin homogenates were measured by ELISA (n = 6). (D) The mRNA levels of M2 markers including Ym-1 , Cd-301 , Dectin-1 , Arg-1 , Irf-4 and Ppar-γ in skin tissues were measured by qPCR (n = 6). (E) The protein levels of M1 markers including p-STAT-1, STAT1, p-p65, p65, NLRP3, p-IκB, IκB, Pro Caspase-1 and ASC in skin tissues were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Figure Legend Snippet: Sauchinone normalizes TGR5/PKA/CREB pathway and regulates M1/M2 macrophages markers in skin of IMQ-induced psoriasis mice. (A) The protein levels of TGR5, PKA. p-CREB, CREB in skin tissues were detected by western blot. (B) Cytokines including IL-6, IL-12 TNF-α, IFN-γ, IL-17 and IL-10 levels in serum were measured by ELISA (n = 6). (C) Cytokines including IL-6, IL-12, TNF-α, IFN-γ, IL-1β and IL-10 levels in skin homogenates were measured by ELISA (n = 6). (D) The mRNA levels of M2 markers including Ym-1 , Cd-301 , Dectin-1 , Arg-1 , Irf-4 and Ppar-γ in skin tissues were measured by qPCR (n = 6). (E) The protein levels of M1 markers including p-STAT-1, STAT1, p-p65, p65, NLRP3, p-IκB, IκB, Pro Caspase-1 and ASC in skin tissues were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay

other:

Article Title: Pro-inflammatory and adjuvant functions of toll-like receptor 4 antagonists
Article Snippet: The following antibodies were used: HA (Roche; 3F10), MyD88 (R&D; AF3109), Actin (Sigma; A 5441), ASC (Millipore, clone 2EI-7), caspase-11 (Biolegend, clone Cas11.17D9), caspase-3 (Santa Cruz, H-277), viperin (Biolegend), phospho-Stat-1 (Cell Signaling, clone 58D6).

Article Title: The neurorepellent SLIT2 inhibits LPS-induced proinflammatory signaling in macrophages
Article Snippet: Macrophages are important mediators of immune responses with critical roles in the recognition and clearance of pathogens, as well as in the resolution of inflammation and wound healing.. The neuronal guidance cue SLIT2 has been widely studied for its effects on immune cell functions, most notably directional cell migration.. Recently, SLIT2 has been shown to directly enhance bacterial killing by macrophages, but the effects of SLIT2 on inflammatory activation of macrophages are less known.

Article Title: Pro-inflammatory and anti-cancer functions of toll-like receptor 4 antagonists
Article Snippet: The following antibodies were used: HA (Roche; 3F10), MyD88 (R&D; AF3109), Actin (Sigma; A 5441), ASC (Millipore, clone 2EI-7), caspase-11 (Biolegend, clone Cas11.17D9), caspase-3 (Santa Cruz, H-277), viperin (Biolegend), phospho-Stat-1 (Cell Signaling, clone 58D6).

Article Title: Pro-inflammatory and anti-cancer functions of toll-like receptor 4 antagonists
Article Snippet: The following antibodies were used: HA (Roche; 3F10), MyD88 (R&D; AF3109), Actin (Sigma; A 5441), ASC (Millipore, clone 2E1-7), caspase-11 (Biolegend, clone Cas11.17D9), caspase-3 (Santa Cruz, H-277), viperin (Biolegend), phospho-Stat-1 (Cell Signaling, clone 58D6).

Software:

Article Title: MBTPS1 regulates proliferation of colorectal cancer primarily through its action on sterol regulatory element-binding proteins
Article Snippet: .. The following antibodies were used: Rabbit polyclonal anti phospho-STAT-1 (Cat. #9167, clone 58D6) from Cell Signaling Technology, and Mouse monoclonal anti-Actin (clone 4) from MP Biomedicals, and Mouse monoclonal anti-SREBP1 (2A4, Cat# SC-13551) from Santa Cruz Biotechnology Inc. Proteins were visualized by a WesternBright ECL (AdvanstaMenlo Park, CA, USA), quantified using Amersham Imager 600 (GE, Buckinghamshire, UK) and analyzed using Quantity One -1D analysis software. .. CRISPR/cas9 mediated knockout of the MBTPS1 gene in HT-29 cells was carried out as following: a guide RNA (gRNA) targeting a coding region in exon 5 of MBTPS1 (5’-ATCGTCCAGCGTTCGCTCGT-3’) was designed using the Optimized CRISPR Design online tool ( http://crispr.mit.edu ) and cloned into pSpCas9(BB)−2A-GFP (PX458), a gift from Feng Zhang (Addgene plasmid #48138) ( ).

Article Title: MBTPS1 regulates proliferation of colorectal cancer primarily through its action on sterol regulatory element-binding proteins.
Article Snippet: .. The following antibodies were used: Rabbit polyclonal anti phospho-STAT-1 (Cat. #9167, clone 58D6) from Cell Signaling Technology, and Mouse monoclonal anti-Actin (clone 4) fromMP Biomedicals, and Mouse monoclonal anti-SREBP1 (2A4, Cat# SC-13551) from Santa Cruz Biotechnology Inc. Proteins were visualized by a WesternBright ECL (AdvanstaMenlo Park, CA, USA), quantified using Amersham Imager 600 (GE, Buckinghamshire, UK) and analyzed using Quantity One -1D analysis software. .. CRISPR/cas9 mediated knockout of the MBTPS1 gene in HT-29 cells was carried out as following: a guide RNA Frontiers in Oncology 04 (gRNA) targeting a coding region in exon 5 of MBTPS1 (5’- ATCGTCCAGCGTTCGCTCGT-3’) was designed using the Optimized CRISPR Design online tool (http://crispr.mit.edu) and cloned into pSpCas9(BB)−2A-GFP (PX458), a gift from Feng Zhang (Addgene plasmid #48138) (32).

Incubation:

Article Title: Paracrine regulations of IFN-γ secreting CD4 + T cells by lumican and biglycan are protective in allergic contact dermatitis
Article Snippet: Protein concentration was quantified by Coomassie Blue protein assay, and 60 μg of lysates were separated on NuPAGE 4–12%, Bis-Tris gel (Invitrogen) with NuPAGE MOPS SDS running buffer (Invitrogen). .. The gels were blotted onto PVDF membranes using a semi-dry apparatus, blocked, and incubated with primary antibodies (1:1000): anti-lumican, -biglycan, phospho-STAT-1, -STAT-1, and -β-actin (Cell Signaling) and anti-β2-integrin (CD18) antibody from BioLegend. ..

Article Title: Paracrine regulations of IFN-γ secreting CD4 + T cells by lumican and biglycan are protective in allergic contact dermatitis.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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